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Image Search Results
Journal: International journal of cancer
Article Title: Proliferation and Cdk4 expression in microsatellite unstable colon cancers with TGFBR2 mutations.
doi: 10.1002/ijc.21399
Figure Lengend Snippet: FIGURE 3 – Representative examples of immunohistochemical staining for cdk4 (a), cyclin D1 (b) and p27Kip1 (c). (a) Representative examples of low and high immunostaining for cdk4. (b) Representa- tive examples of low and high levels of immunostaining for cyclin D1. (c) Representative examples of immunostaining for p27Kip1. Cyto- plasmic as well as nuclear staining can be appreciated.
Article Snippet: Expression of cyclin D1, cdk4, p27 and Ki-67 was detected by immunohistochemistry using the following antibodies: cyclin D1DCS-6 [mouse monoclonal antibody (mAb); Neomarkers, Freemont, CA],
Techniques: Immunohistochemical staining, Staining, Immunostaining
Journal: International journal of cancer
Article Title: Proliferation and Cdk4 expression in microsatellite unstable colon cancers with TGFBR2 mutations.
doi: 10.1002/ijc.21399
Figure Lengend Snippet: FIGURE 4 – (a) Results of 3TP Lux assay on HCT116/Chr3 and HCT116/Chr3 1 DN2R. Luciferase activity from the 3TP-Lux reporter was assessed after 48 hr of treatment with TGF-b (10 ng/ml). The HCT116/Chr3 cells show a 163 increase in luciferase activity after TGF-b treat- ment compared to the HCT116/Chr3 1 DN2R cells, which show a 53 increase in luciferase activity after TGF-b treatment (Student’s t-test; HCT116/Chr3 1 TGF-b vs. HCT116/Chr3 1 TGF-b; p 5 0.0009). (b) Expression of cdk4 in HCT116/Chr3 after transfection with dominant negative TGFBR2 (DN2R). Cdk4 expression is increased in the HCT116/Chr3 1 DN2R cell line compared to the HCT116/Chr3 line. TGF-b modestly suppresses cdk4 expression in the HCT116/Chr3 cells but does not suppress cdk4 expression in the HCT116/Chr3 1 DN2R line. (c) Cdk4-mediated phosphorylation of Rb on Ser249/Thr252 (pRb) is reduced in the HCT116/Chr3 compared to HCT116/Chr3 1 DN2R. There is no substantial difference in the expression of Rb between HCT116/Chr3 and HCT116/Chr3 1 DN2R.
Article Snippet: Expression of cyclin D1, cdk4, p27 and Ki-67 was detected by immunohistochemistry using the following antibodies: cyclin D1DCS-6 [mouse monoclonal antibody (mAb); Neomarkers, Freemont, CA],
Techniques: Luciferase, Activity Assay, Expressing, Transfection, Dominant Negative Mutation, Phospho-proteomics
Journal: Journal of Clinical and Experimental Dentistry
Article Title: Juvenile trabecular ossifying fibroma: Immunohistochemical expression of MDM2, CDK4 and p53 compared to conventional ossifying fibroma
doi: 10.4317/jced.59116
Figure Lengend Snippet: a) Immunostaining for CDK4 in a JOF case showing a diffuse and strong staining pattern. b) Moderate MDM2 immunoexpression in a JOF case. c) Focal p53 immunoexpression in a JOF case (400X).
Article Snippet: After extensive washing, the sections then were incubated for 1 hour with primary antibody: MDM2 (Santa Cruz Biotechnology, SMP14, sc-965) 1:50,
Techniques: Immunostaining, Staining
Journal: Journal of Clinical and Experimental Dentistry
Article Title: Juvenile trabecular ossifying fibroma: Immunohistochemical expression of MDM2, CDK4 and p53 compared to conventional ossifying fibroma
doi: 10.4317/jced.59116
Figure Lengend Snippet: Bar graph demonstrating the percentage of positive JOF and OF cases for CDK4, MDM2 and p53. For comparison, the percentage of OS cases demonstrating positivity for the same markers is also included, based on previously published data by our group .
Article Snippet: After extensive washing, the sections then were incubated for 1 hour with primary antibody: MDM2 (Santa Cruz Biotechnology, SMP14, sc-965) 1:50,
Techniques: Comparison